Functional Investigation of Iron-Responsive Microsomal Proteins, including MirC, in Aspergillus fumigatus.

ABSTRACT: The functionality of many microsome-associated proteins which exhibit altered abundance in response to iron limitation in Aspergillus fumigatus is unknown. Here, we generate and characterize eight gene deletion strains, and of most significance reveal that MirC (AFUA_2G05730) contributes to the maintenance of intracellular siderophore [ferricrocin (FC)] levels, augments conidiation, confers protection against oxidative stress, exhibits an intracellular localization and contributes to fungal virulence in the Galleria mellonella animal model system. FC levels were unaffected following deletion of all other genes encoding microsome-associated proteins. MirC does not appear to play a role in either siderophore export from, or uptake into, A. fumigatus. Label-free quantitat

SUBMITTER: Mulvihill ED 

PROVIDER: S-EPMC5355445 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature