The architecture of chicken chromosome territories changes during differentiation.

ABSTRACT:

Background

Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown.

Results

We compared the large-scale chromatin structure of chromosome territories between several hematopoietic chicken cell types at various differentiation stages. Chromosome territories were labeled by fluorescence in situ hybridization in structurally preserved nuclei, recorded by confocal microscopy and evaluated visually and by quantitative image analysis. Chromosome territories in multipotent myeloid precursor cells appeared homogeneously stained and compact. The inactive lysozyme gene as well as the centromere of the lysozyme

SUBMITTER: Stadler S 

PROVIDER: S-EPMC535556 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature