Asporin is a stromally expressed marker associated with prostate cancer progression.

ABSTRACT:

Background

Prostate cancer shows considerable heterogeneity in disease progression and we propose that markers expressed in tumour stroma may be reliable predictors of aggressive tumour subtypes.

Methods

We have used Kaplan-Meier, univariate and multivariate analysis to correlate the expression of Asporin (ASPN) mRNA and protein with prostate cancer progression in independent cohorts. We used immunohistochemistry and H scoring to document stromal localisation of ASPN in a tissue microarray and mouse prostate cancer model, and correlated expression with reactive stroma, defined using Masson Trichrome staining. We used cell cultures of primary prostate cancer fibroblasts treated with serum-free conditioned media from prostate cancer cell lines to examine regulation of ASPN mRN

SUBMITTER: Rochette A 

PROVIDER: S-EPMC5355923 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature