Copper transporters are responsible for copper isotopic fractionation in eukaryotic cells.

ABSTRACT: Copper isotopic composition is altered in cancerous compared to healthy tissues. However, the rationale for this difference is yet unknown. As a model of Cu isotopic fractionation, we monitored Cu uptake in Saccharomyces cerevisiae, whose Cu import is similar to human. Wild type cells are enriched in ⁶³Cu relative to ⁶⁵Cu. Likewise, ⁶³Cu isotope enrichment in cells without high-affinity Cu transporters is of slightly lower magnitude. In cells with compromised Cu reductase activity, however, no isotope fractionation is observed and when Cu is provided solely in reduced form for this strain, copper is enriched in ⁶³Cu like in the case of the wild type. Our results demonstrate that Cu isotope fractionation is generated by membrane importers and that

SUBMITTER: Cadiou JL 

PROVIDER: S-EPMC5356015 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature