Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date.

ABSTRACT:

Background

Floudas et al. (Science 336: 1715) established that lignin-degrading fungi appeared at the end of Carboniferous period associated with the production of the first ligninolytic peroxidases. Here, the subsequent evolution of these enzymes in Polyporales, where most wood-rotting fungi are included, is experimentally recreated using genomic information.

Results

With this purpose, we analyzed the evolutionary pathway leading to the most efficient lignin-degrading peroxidases characterizing Polyporales species. After sequence reconstruction from 113 genes of ten sequenced genomes, the main enzyme intermediates were resurrected and characterized. Biochemical changes were analyzed together with predicted sequences and structures, to understand how these enzymes acq

SUBMITTER: Ayuso-Fernandez I 

PROVIDER: S-EPMC5356311 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature