The microbiome composition of Aedes aegypti is not critical for Wolbachia-mediated inhibition of dengue virus.

ABSTRACT:

Background

Dengue virus (DENV) is primarily vectored by the mosquito Aedes aegypti, and is estimated to cause 390 million human infections annually. A novel method for DENV control involves stable transinfection of Ae. aegypti with the common insect endosymbiont Wolbachia, which mediates an antiviral effect. However, the mechanism by which Wolbachia reduces the susceptibility of Ae. aegypti to DENV is not fully understood. In this study we assessed the potential of resident microbiota, which can play important roles in insect physiology and immune responses, to affect Wolbachia-mediated DENV blocking.

Methodology/findings

The microbiome of Ae. aegypti stably infected with Wolbachia strain wMel was compared to that of Ae. aegypti without Wolbachia, using 16s rDNA profiling. O

SUBMITTER: Audsley MD 

PROVIDER: S-EPMC5357062 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature