Landscape of monoallelic DNA accessibility in mouse embryonic stem cells and neural progenitor cells.

ABSTRACT: We developed an allele-specific assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) to genotype and profile active regulatory DNA across the genome. Using a mouse hybrid F₁ system, we found that monoallelic DNA accessibility across autosomes was pervasive, developmentally programmed and composed of several patterns. Genetically determined accessibility was enriched at distal enhancers, but random monoallelically accessible (RAMA) elements were enriched at promoters and may act as gatekeepers of monoallelic mRNA expression. Allelic choice at RAMA elements was stable across cell generations and bookmarked through mitosis. RAMA elements in neural progenitor cells were biallelically accessible in embryonic stem cells but premarked with bivalent hist

SUBMITTER: Xu J 

PROVIDER: S-EPMC5357084 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature