Tumor-induced alterations in lymph node lymph drainage identified by contrast-enhanced MRI.

ABSTRACT:

Background

To use high resolution MRI lymphography to characterize altered tumor-draining lymph node (TDLN) lymph drainage in response to growth of aggressive tumors.

Methods

Six mice bearing B16-F10 melanomas in one rear footpad were imaged by 3.0 Tesla (T) MRI before and after subcutaneous injection of Gadofosveset trisodium (Gd-FVT) contrast agent into both rear feet. Gd-FVT uptake into the left and right draining popliteal LNs was quantified and compared using Wilcoxon signed-rank test. Fluorescent dextran lymphography compared patterns of LN lymph drainage with the pattern of immunostained lymphatic sinuses by fluorescence microscopy.

Results

TDLNs exhibited greater Gd-FVT uptake than contralateral uninvolved LNs, although this difference did not reach significan

SUBMITTER: Ruddell A 

PROVIDER: S-EPMC5357140 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature