Sets of serum exosomal microRNAs as candidate diagnostic biomarkers for Kawasaki disease.

ABSTRACT: Although Kawasaki disease is the main cause of acquired heart disease in children, no diagnostic biomarkers are available. We aimed to identify candidate biomarkers for diagnosing Kawasaki disease using serum exosomal microRNAs (miRNAs). Using frozen serum samples from a biobank, high-throughput microarray technologies, two-stage real-time quantitative PCR, and a self-referencing strategy for data normalization, we narrowed down the list of biomarker candidates to a set of 4 miRNAs. We further validated the diagnostic capabilities of the identified miRNAs (namely, C_T(miR-1246)-C_T(miR-4436b-5p) and C_T(miR-197-3p)-C_T(miR-671-5p)) in 79 samples from two hospitals. We found that this 4-miRNA set could distinguish KD patients from other febrile patien

SUBMITTER: Jia HL 

PROVIDER: S-EPMC5357789 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature