Priming of transcriptional memory responses via the chromatin accessibility landscape in T cells.

ABSTRACT: Memory T cells exhibit transcriptional memory and "remember" their previous pathogenic encounter to increase transcription on re-infection. However, how this transcriptional priming response is regulated is unknown. Here we performed global FAIRE-seq profiling of chromatin accessibility in a human T cell transcriptional memory model. Primary activation induced persistent accessibility changes, and secondary activation induced secondary-specific opening of previously less accessible regions associated with enhanced expression of memory-responsive genes. Increased accessibility occurred largely in distal regulatory regions and was associated with increased histone acetylation and relative H3.3 deposition. The enhanced re-stimulation response was linked to the strength of initial PKC-induced

SUBMITTER: Tu WJ 

PROVIDER: S-EPMC5357947 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature