Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis.

ABSTRACT: Type VI secretion system (T6SS) is a versatile protein export machinery widely distributed in Gram-negative bacteria. Known to translocate protein substrates to eukaryotic and prokaryotic target cells to cause cellular damage, the T6SS has been primarily recognized as a contact-dependent bacterial weapon for microbe-host and microbial interspecies competition. Here we report contact-independent functions of the T6SS for metal acquisition, bacteria competition, and resistance to oxidative stress. We demonstrate that the T6SS-4 in Burkholderia thailandensis is critical for survival under oxidative stress and is regulated by OxyR, a conserved oxidative stress regulator. The T6SS-4 is important for intracellular accumulation of manganese (Mn²⁺) under oxidative stress. Next, w

SUBMITTER: Si M 

PROVIDER: S-EPMC5358365 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature