Rapid and efficient generation of oligodendrocytes from human induced pluripotent stem cells using transcription factors.

ABSTRACT: Rapid and efficient protocols to generate oligodendrocytes (OL) from human induced pluripotent stem cells (iPSC) are currently lacking, but may be a key technology to understand the biology of myelin diseases and to develop treatments for such disorders. Here, we demonstrate that the induction of three transcription factors (SOX10, OLIG2, NKX6.2) in iPSC-derived neural progenitor cells is sufficient to rapidly generate O4⁺ OL with an efficiency of up to 70% in 28 d and a global gene-expression profile comparable to primary human OL. We further demonstrate that iPSC-derived OL disperse and myelinate the CNS of Mbp^shi/shiRag^-/- mice during development and after demyelination, are suitable for in vitro myelination assays, disease mo

SUBMITTER: Ehrlich M 

PROVIDER: S-EPMC5358375 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature