Natural variation and dosage of the HEI10 meiotic E3 ligase control <i>Arabidopsis</i> crossover recombination.

ABSTRACT: During meiosis, homologous chromosomes undergo crossover recombination, which creates genetic diversity and balances homolog segregation. Despite these critical functions, crossover frequency varies extensively within and between species. Although natural crossover recombination modifier loci have been detected in plants, causal genes have remained elusive. Using natural Arabidopsis thaliana accessions, we identified two major recombination quantitative trait loci (rQTLs) that explain 56.9% of crossover variation in Col×Ler F₂ populations. We mapped rQTL1 to semidominant polymorphisms in HEI10, which encodes a conserved ubiquitin E3 ligase that regulates crossovers. Null hei10 mutants are haploinsufficient, and, using genome-wide mapping and im

SUBMITTER: Ziolkowski PA 

PROVIDER: S-EPMC5358726 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature