Predictive Modeling of a Batch Filter Mating Process.

ABSTRACT: Quantitative characterizations of horizontal gene transfer are needed to accurately describe gene transfer processes in natural and engineered systems. A number of approaches to the quantitative description of plasmid conjugation have appeared in the literature. In this study, we seek to extend that work, motivated by the question of whether a mathematical model can accurately predict growth and conjugation dynamics in a batch process. We used flow cytometry to make time-point observations of a filter-associated mating between two E. coli strains, and fit ordinary differential equation models to the data. A model comparison analysis identified the model formulation that is best supported by the data. Identifiability analysis revealed that the parameters were estimated with acceptabl

SUBMITTER: Malwade A 

PROVIDER: S-EPMC5359259 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature