Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.

ABSTRACT: Quantitative PCR (qPCR) is an important tool in pathogen detection. However, the use of different qPCR components, calibration materials and DNA extraction methods reduces comparability between laboratories, which can result in false diagnosis and discrepancies in patient care. The wider establishment of a metrological framework for nucleic acid tests could improve the degree of standardisation of pathogen detection and the quantification methods applied in the clinical context. To achieve this, accurate methods need to be developed and implemented as reference measurement procedures, and to facilitate characterisation of suitable certified reference materials. Digital PCR (dPCR) has already been used for pathogen quantification by analysing nucleic acids. Although dPCR has the potential t

SUBMITTER: Pavsic J 

PROVIDER: S-EPMC5359388 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature