Development of oriC-Based Plasmids for Mesoplasma florum.

ABSTRACT: The near-minimal bacterium Mesoplasma florum constitutes an attractive model for systems biology and for the development of a simplified cell chassis in synthetic biology. However, the lack of genetic engineering tools for this microorganism has limited our capacity to understand its basic biology and modify its genome. To address this issue, we have evaluated the susceptibility of M. florum to common antibiotics and developed the first generation of artificial plasmids able to replicate in this bacterium. Selected regions of the predicted M. florum chromosomal origin of replication (oriC) were used to create different plasmid versions that were tested for their transformation frequency and stability. Using polyethylene glycol-mediated transformation, we observe

SUBMITTER: Matteau D 

PROVIDER: S-EPMC5359490 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature