Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species.

ABSTRACT: Synthetic biology and metabolic engineering experiments frequently require the fine-tuning of gene expression to balance and optimize protein levels of regulators or metabolic enzymes. A key concept of synthetic biology is the development of modular parts that can be used in different contexts. Here, we have applied a computational multifactor design approach to generate de novo synthetic core promoters and 5' untranslated regions (UTRs) for yeast cells. In contrast to upstream cis-regulatory modules (CRMs), core promoters are typically not subject to specific regulation, making them ideal engineering targets for gene expression fine-tuning. 112 synthetic core promoter sequences were designed on the basis of the sequence/function relationship of natural core promoters, nucleosome occupancy

SUBMITTER: Portela RM 

PROVIDER: S-EPMC5359585 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature