A new electro-optical approach for conductance measurement: an assay for the study of drugs acting on ligand-gated ion channels.

ABSTRACT: Ligand gated ion channels are involved in many pathophysiological processes and represent a relevant, although challenging, target for drug discovery. We propose an innovative electro-optical approach to their analysis able to derive membrane conductance values from the local membrane potential changes imposed by test current pulses and measured by fast voltage-sensitive fluorescent dyes. We exploited the potential of this proprietary method by developing a drug testing system called "ionChannel Optical High-content Microscope" (ionChannelΩ). This automated platform was validated by testing the responses of reference drugs on cells expressing different ligand-gated ion channels. Furthermore, a double-blind comparison with FLIPR and automated patch-clamp was performed on molecules designed

SUBMITTER: Menegon A 

PROVIDER: S-EPMC5359596 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature