H₂S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression.

ABSTRACT: The aims of the present study are to determine whether hydrogen sulfide (H₂S) is involved in the expression of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) production, and to identify the role of microRNA-455-3p (miR-455-3p) during those processes. In cultured human umbilical vein endothelial cells (HUVECs), the expression of miR-455-3p, eNOS protein and the NO production was detected after administration with 50 μM NaHS. The results indicated that H₂S could augment the expression of miR-455-3p and eNOS protein, leading to the increase of NO level. We also found that overexpression of miR-455-3p in HUVECs increased the protein levels of eNOS whereas inhibition of miR-455-3p decreased it. Moreover, H₂S and miR-455-3p could no longer increas

SUBMITTER: Li XH 

PROVIDER: S-EPMC5359669 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature