Establishment of transient gene expression systems in protoplasts from Liriodendron hybrid mesophyll cells.

ABSTRACT: Liriodendron is a genus of the magnolia family comprised of two flowering tree species that produce hardwoods of great ecological and economic value. However, only a limited amount of genetic research has been performed on the Liriodendron genus partly because transient or stable transgenic trees have been difficult to produce. In general, transient expression systems are indispensable for rapid, high-throughput screening and systematic characterization of gene functions at a low cost; therefore, development of such a system for Liriodendron would provide a necessary step forward for research on Magnoliaceae and other woody trees. Herein, we describe an efficient and rapid protocol for preparing protoplasts from the leaf mesophyll tissue of a Liriodendron hybrid and an optimized system for

SUBMITTER: Huo A 

PROVIDER: S-EPMC5360215 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature