Reestablishment of Glucose Inhibition of Glucagon Secretion in Small Pseudoislets.

ABSTRACT: Misregulated hormone secretion from the islet of Langerhans is central to the pathophysiology of diabetes. Although insulin plays a key role in glucose regulation, the importance of glucagon is increasingly acknowledged. However, the mechanisms that regulate glucagon secretion from α-cells are still unclear. We used pseudoislets reconstituted from dispersed islet cells to study α-cells with and without various indirect effects from other islet cells. Dispersed islet cells secrete aberrant levels of glucagon and insulin at basal and elevated glucose levels. When cultured, murine islet cells reassociate to form pseudoislets, which recover normal glucose-regulated hormone secretion, and human islet cells follow a similar pattern. We created small (∼40-µm) pseudoislets using all of the islet c

SUBMITTER: Reissaus CA 

PROVIDER: S-EPMC5360306 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature