CDKL1 promotes tumor proliferation and invasion in colorectal cancer.

ABSTRACT:

Background

CDKL1 is a member of the cell division cycle 2 (CDC2)-related serine threonine protein kinase family and is overexpressed in malignant tumors such as melanoma, breast cancer, and gastric cancer.

Objective

This study aimed to evaluate whether CDKL1 can serve as a potential molecular target for colorectal cancer therapy.

Materials and methods

Expression of CDKL1 in colorectal cancer tissues and cell lines was measured by immunohistochemistry and Western blot, respectively. To investigate the role of CDKL1 in colorectal cancer, CDKL1-small hairpin RNA-expressing lentivirus was constructed and infected into HCT116 and Caco2 cells. The effects of RNA interference (RNAi)-mediated CDKL1 downregulation on cell proliferation and invasion were assessed by CCK-8, colo

SUBMITTER: Qin C 

PROVIDER: S-EPMC5360398 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature