Differential responses of choroidal melanocytes and uveal melanoma cells to low oxygen conditions.

ABSTRACT:

Purpose

Tissue culture is traditionally performed at atmospheric oxygen concentration (21%), which induces hyperoxic stress, as endogenous physiologic oxygen tension found in tissues varies between 2% and 9%. This discrepancy may lead to misinterpretation of results and may explain why effects observed in vitro cannot always be reproduced in vivo and vice versa. Only a few studies have been conducted in low physiologic oxygen conditions to understand the development and differentiation of cells from the eye.

Methods

The aim of this study was to investigate the growth and gene expression profile of melanocytes from the choroid permanently exposed to 21% (hyperoxic) or 3% (physiologic) oxygen with proliferation assays and DNA microarray. The cellular behavior of the melanocyte

SUBMITTER: Weidmann C 

PROVIDER: S-EPMC5360455 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature