Osmotic regulation of NFAT5 expression in RPE cells: The involvement of purinergic receptor signaling.

ABSTRACT:

Purpose

Systemic hypertension is a risk factor for age-related neovascular retinal diseases. The major condition that induces hypertension is the intake of dietary salt (NaCl) resulting in increased extracellular osmolarity. High extracellular NaCl was has been shown to induce angiogenic factor production in RPE cells, in part via the transcriptional activity of nuclear factor of activated T cell 5 (NFAT5). Here, we determined the signaling pathways that mediate the osmotic expression of the NFAT5 gene in RPE cells.

Methods

Cultured human RPE cells were stimulated with high (+100 mM) NaCl. Alterations in gene and protein expression were determined with real-time reverse transcriptase (RT)-PCR and western blot analysis, respectively.

Results

NaCl-induced NFAT

SUBMITTER: Hollborn M 

PROVIDER: S-EPMC5360457 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature