Real-time detection and single-pass minimization of TEM objective lens astigmatism.

ABSTRACT: Minimization of the astigmatism of the objective lens is a critical daily instrument alignment task essential for high resolution TEM imaging. Fast and sensitive detection of astigmatism is needed to provide real-time feedback and adjust the stigmators to efficiently reduce astigmatism. Currently the method used by many microscopists is to visually examine the roundness of a diffractogram (Thon rings) and iteratively adjust the stigmators to make the Thon rings circular. This subjective method is limited by poor sensitivity and potentially biased by the astigmatism of human eyes. In this study, an s² power spectra based method, s²stigmator, was developed to allow fast and sensitive detection of the astigmatism in TEM live images. The "radar"-style display provides rea

SUBMITTER: Yan R 

PROVIDER: S-EPMC5360486 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature