Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins.

ABSTRACT: The ability to site-specifically incorporate non-canonical amino acids (ncAAs) into proteins has made possible the study of protein structure and function in fundamentally new ways, as well as the bio synthesis of unnatural polymers. However, the task of site-specifically incorporating multiple ncAAs into proteins with high purity and yield continues to present a challenge. At the heart of this challenge lies the lower efficiency of engineered orthogonal translation system components compared to their natural counterparts (e.g., translation elements that specifically use a ncAA and do not interact with the cell's natural translation apparatus). Here, we show that evolving and tuning expression levels of multiple components of an engineered translation system together as a whole enhances nc

SUBMITTER: Gan R 

PROVIDER: S-EPMC5360495 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature