Bovine in vitro embryo production: the effects of fibroblast growth factor 10 (FGF10).

ABSTRACT:

Purpose

In an attempt to improve in vitro embryo production, we investigated the effect of fibroblast growth factor 10 (FGF10) during in vitro maturation on the developmental capacity of bovine oocytes.

Material and methods

Cumulus-oocyte complexes (COCs) were aspirated from follicles of 3-8 mm diameter. After selection, the COCs were matured in medium with or without 0.5 ng/mL of FGF10. The effect of FGF10 during in vitro maturation (IVM) on nuclear maturation kinetics and expansion of the cumulus cells was investigated. Oocyte competence was assessed by the production and development speed of embryos and the relative expression of genes associated with embryo quality.

Results

FGF10 delayed the resumption of meiosis from 8 h onwards, but did not affect the percentage

SUBMITTER: Diogenes MN 

PROVIDER: S-EPMC5360681 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature