Transient Bluetongue virus serotype 8 capsid protein expression in Nicotiana benthamiana.

ABSTRACT: Bluetongue virus (BTV) causes severe disease in domestic and wild ruminants, and has recently caused several outbreaks in Europe. Current vaccines include live-attenuated and inactivated viruses; while these are effective, there is risk of reversion to virulence by mutation or reassortment with wild type viruses. Subunit or virus-like particle (VLP) vaccines are safer options: VLP vaccines produced in insect cells by expression of the four BTV capsid proteins are protective against challenge; however, this is a costly production method. We investigated production of BTV VLPs in plants via Agrobacterium-mediated transient expression, an inexpensive production system very well suited to developing country use. Leaves infiltrated with recombinant pEAQ-HT vectors separately encod

SUBMITTER: van Zyl AR 

PROVIDER: S-EPMC5360979 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature