8-Styryl-substituted coralyne derivatives as DNA binding fluorescent probes.

ABSTRACT: Six new 8-styryl-substituted coralyne derivatives 4a-f were synthesized from coralyne (2) by a base catalysed Knoevenagel type reaction. It was shown by photometric and fluorimetric titrations of double stranded and quadruplex DNA to 4b-d as well as by fluorimetric DNA denaturation experiments that these ligands bind to DNA with different binding modes at varying ligand-DNA ratios (LDR). Specifically, the addition of DNA caused initially a hypochromic effect in absorbance and, at a particular LDR, the development of a new red shifted absorption band with a hyperchromic effect. Furthermore, 4b-d induced a significant and selective stabilization of quadruplex DNA towards unfolding (ΔT_m = 31.6-32.9 °C at LDR = 5), which is even more pronounced as

SUBMITTER: Pithan PM 

PROVIDER: S-EPMC5361113 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature