Combinatorial selection for replicable RNA by Qβ replicase while maintaining encoded gene function.

ABSTRACT: Construction of a complex artificial self-replication system is challenging in the field of in vitro synthetic biology. Recently, we developed a translation-coupled RNA replication system, wherein an artificial genomic RNA replicates with the Qβ RNA replicase gene encoded on itself. The challenge is to introduce additional genes into the RNA to develop a complex system that mimics natural living systems. However, most RNA sequence encoding genes are not replicable by the Qβ replicase owing to its requirement for strong secondary structures throughout the RNA sequence that are absent in most genes. In this study, we establish a new combinatorial selection method to find an RNA sequence with secondary structures and functional amino acid sequences of the encoded gene. We selected RNA sequenc

SUBMITTER: Yumura M 

PROVIDER: S-EPMC5362092 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature