Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip.

ABSTRACT: Portable, low-cost, and quantitative nucleic acid detection is desirable for point-of-care diagnostics; however, current polymerase chain reaction testing often requires time-consuming multiple steps and costly equipment. We report an integrated microfluidic diagnostic device capable of on-site quantitative nucleic acid detection directly from the blood without separate sample preparation steps. First, we prepatterned the amplification initiator [magnesium acetate (MgOAc)] on the chip to enable digital nucleic acid amplification. Second, a simplified sample preparation step is demonstrated, where the plasma is separated autonomously into 224 microwells (100 nl per well) without any hemolysis. Furthermore, self-powered microfluidic pumping without any external pumps, controllers, or power s

SUBMITTER: Yeh EC 

PROVIDER: S-EPMC5362183 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature