MiRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole.

ABSTRACT:

Purpose

The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group.

Methods

In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in consecutive patients who underwent standard vitrectomy for ERM and MH. RNA was extracted and TaqMan® Low Density Arrays (TLDAs) were used to profile the transcriptome of 754 miRNAs. Results were validated by single TaqMan® assays. Finally, we created a biological network of differentially expressed miRNA targets and their nearest neighbors.

Results

Overall 10 eyes with MH, 16 eyes with idiopathic ERM and 6 controls were enrolled in the study. Profiling data ident

SUBMITTER: Russo A 

PROVIDER: S-EPMC5362220 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature