A novel recombinant variant of latent membrane protein 1 from Epstein Barr virus in Argentina denotes phylogeographical association.

ABSTRACT:

Aim

To study LMP1 variants distribution among children with EBV+ malignant and benign conditions as well as in healthy carriers.

Methods

Oral secretions and blood cells from 31 children with IM, and biopsies from 14 EBV+ reactive lymphoid hyperplasia and 33 EBV+ lymphomas were included. LMP1 was amplified by nested PCR and sequenced. Phylogenetic reconstructions were made under Maximun Likelihood, Bayesian and coalescent algorithms.

Results

Six clades were defined (China1, China2, Med-, Alaskan, B95.8 and Argentine). Argentine variants, the most prevalent (46%), harbored 3 distinctive mutations and were a recombination between Raji and China1. Despite no pathology or compartment associations were observed for LMP1, the Argentine clade showed a phylogeographic associat

SUBMITTER: Gantuz M 

PROVIDER: S-EPMC5362222 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature