Ligand-promoted protein folding by biased kinetic partitioning.
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ABSTRACT:
SUBMITTER: Hingorani KS
PROVIDER: S-EPMC5362304 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Publications
Ligand-promoted protein folding by biased kinetic partitioning.
Hingorani Karan S KS Metcalf Matthew C MC Deming Derrick T DT Garman Scott C SC Powers Evan T ET Gierasch Lila M LM
Nature chemical biology 20170220 4
Protein folding in cells occurs in the presence of high concentrations of endogenous binding partners, and exogenous binding partners have been exploited as pharmacological chaperones. A combined mathematical modeling and experimental approach shows that a ligand improves the folding of a destabilized protein by biasing the kinetic partitioning between folding and alternative fates (aggregation or degradation). Computationally predicted inhibition of test protein aggregation and degradation as a ...[more]
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