Selective disulfide reduction for labeling and enhancement of Fab antibody fragments.

ABSTRACT: Many methods have been developed for chemical labeling and enhancement of the properties of antibodies and their common fragments, including the Fab and F(ab')₂ fragments. Somewhat selective reduction of some antibody disulfide bonds has been previously achieved, yielding antibodies and antibody fragments that can be labeled at defined sites, enhancing their utility and properties. Selective reduction of the two hinge disulfide bonds present in F(ab')₂ fragments using mild reduction has been useful. However, such reduction is often not quantitative and results in the reduction of multiple disulfide bonds, and therefore subsequent multiple labeling or conjugation sites are neither homogenous nor stoichiometric. Here, a simple and efficient selective reduction of the si

SUBMITTER: Kirley TL 

PROVIDER: S-EPMC5362313 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature