FAT4 hypermethylation and grade dependent downregulation in gastric adenocarcinoma.

ABSTRACT: Gastric cancer is one of the major causes of death due to cancer in the world. It is a multi-factorial disease with epigenetic factors being also involved in its development. FAT4 is a tumor suppressor gene exerting an important role in cell adhesion. This study aimed at analyzing FAT4 expression and promoter methylation in gastric cancer. FAT4 expression was studied in 30 tumoral tissues and their non-tumoral counterparts using Taqman real time PCR method. Promoter methylation was assessed using bisulfite conversion method followed by sequencing. Tumor tissues showed reduced FAT4 expression (P = 0.04). FAT4 downregulation was associated with tumor grade, with higher repression at advanced grades. Significant increase of promoter methylation was observed in tumoral tissues. Reduced express

SUBMITTER: Pilehchian Langroudi M 

PROVIDER: S-EPMC5362569 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature