Virulence and Genomic Feature of a Virulent Klebsiella pneumoniae Sequence Type 14 Strain of Serotype K2 Harboring bla_NDM-5 in China.

ABSTRACT: The objective of this study was to reveal the molecular mechanism involved in carbapenem resistance and virulence of a K2 Klebsiella pneumoniae clinical isolate 24835. The virulence of the strain was determined by in vitro and in vivo methods. The de novo whole-genome sequencing technology and molecular biology methods were used to analyze the genomic features associated with the carbapenem resistance and virulence of K. pneumoniae 24835. Strain 24835 was highly resistant to carbapenems and belonged to ST14, exhibited hypermucoviscous and unique K2-aerobactin-kfu-rmpA positive phenotype. As the only carbapenemase gene in strain 24835, bla_NDM-5 was located on a 46-kb IncX3 self-transmissible plasmid, which is a very close relation of pNDM

SUBMITTER: Mei YF 

PROVIDER: S-EPMC5362587 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature