The defence-associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias.

ABSTRACT: Bread wheat (Triticum aestivum L.) is an allopolyploid species containing three ancestral genomes. Therefore, three homoeologous copies exist for the majority of genes in the wheat genome. Whether different homoeologs are differentially expressed (homoeolog expression bias) in response to biotic and abiotic stresses is poorly understood. In this study, we applied a RNA-seq approach to analyse homoeolog-specific global gene expression patterns in wheat during infection by the fungal pathogen Fusarium pseudograminearum, which causes crown rot disease in cereals. To ensure specific detection of homoeologs, we first optimized read alignment methods and validated the results experimentally on genes with known patterns of subgenome-specific expression. Our global analysis identified widespread p

SUBMITTER: Powell JJ 

PROVIDER: S-EPMC5362679 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature