Toward an Optimized Workflow for Middle-Down Proteomics.

ABSTRACT: Mass spectrometry (MS)-based proteomics workflows can crudely be classified into two distinct regimes, targeting either relatively small peptides (i.e., 0.7 kDa < M_w < 3.0 kDa) or small to medium sized intact proteins (i.e., 10 kDa < M_w < 30 kDa), respectively, termed bottom-up and top-down proteomics. Recently, a niche has started to be explored covering the analysis of middle-range peptides (i.e., 3.0 kDa < M_w < 10 kDa), aptly termed middle-down proteomics. Although middle-down proteomics can follow, in principle, a modular workflow similar to that of bottom-up proteomics, we hypothesized that each of these modules would benefit from targeted optimization to improve its overall performance in the analysis of middle-range sized peptides. Hence, to generate

SUBMITTER: Cristobal A 

PROVIDER: S-EPMC5362747 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature