Mechanistic platform knowledge of concomitant sugar uptake in Escherichia coli BL21(DE3) strains.

ABSTRACT: When producing recombinant proteins, the use of Escherichia coli strain BL21(DE3) in combination with the T7-based pET-expression system is often the method of choice. In a recent study we introduced a mechanistic model describing the correlation of the specific glucose uptake rate (q_s,glu) and the corresponding maximum specific lactose uptake rate (q_s,lac,max) for a pET-based E. coli BL21(DE3) strain producing a single chain variable fragment (scFv). We showed the effect of q_s,lac,max on productivity and product location underlining its importance for recombinant protein production. In the present study we investigated the mechanistic q_s,glu/q_s,lac,max correlation for four pET-based E. coli BL21(DE3) strains producing different recomb

SUBMITTER: Wurm DJ 

PROVIDER: S-EPMC5362885 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature