Multiple H⁺ sensors mediate the extracellular acidification-induced [Ca²⁺]_i elevation in cultured rat ventricular cardiomyocytes.

ABSTRACT: Acidosis has been known to cause "Ca²⁺ transients", however, the mechanism is still uncertain. Here, we demonstrated that multiple H⁺ sensors, such as ASICs, TRPV1 and proton-sensing G protein coupled receptors (GPCRs) are involved in extracellular acidification-induced intracellular calcium ([Ca²⁺]_i) elevation. By using calcium imaging measures, we observed that both ASIC and TRPV1 channels inhibitors suppressed the [Ca²⁺]_i elevation induced by extracellular acidosis in cultured rat cardiac myocytes. Then, both channels mRNA and proteins were identified by RT-PCR, western blotting and immunofluorescence. ASIC-like and TRPV1-like currents were induced by extracellular acidification, suggesting that functional ASIC and TRPV1

SUBMITTER: Hu YL 

PROVIDER: S-EPMC5362981 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature