Combination therapy with L-arginine and α-PD-L1 antibody boosts immune response against osteosarcoma in immunocompetent mice.

ABSTRACT: L-arginine supplementation was recently proved to promote the function of immune cells, especially T-cells, by facilitating T-cell proliferation, differentiation and survival in vivo. Cytotoxic CD8⁺ plays a crucial role in modulating anti-cancer response mediated by the immune system, but was restricted by exhaustion. Thus, we hypothesized that L-arginine, in combination with α-PD-L1 antibody, may provide a favored environment for T-cell response against osteosarcoma. Immunocompetent BALB/c mouse models bearing orthotopic and metastatic osteosarcoma were established to validate this conjecture. We found that L-arginine significantly elevated the number of splenic CD8⁺ T-cells, the level of serum interferon-γ, and CD8⁺ T-cell infiltration. Furthermore, α-PD-

SUBMITTER: He X 

PROVIDER: S-EPMC5362985 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature