Uncovering SNP and indel variations of tetraploid cottons by SLAF-seq.

ABSTRACT:

Background

Cotton (Gossypium spp.), as the world's most utilized textile fibre source, is an important, economically valuable crop worldwide. Understanding the genomic variation of tetraploid cotton species is important for exploitation of the excellent characteristics of wild cotton and for improving the diversity of cotton in breeding. However, the discovery of DNA polymorphisms in tetraploid cotton genomes has lagged behind other important crops.

Results

A total of 111,795,823 reads, 467,735 specific length amplified fragment (SLAF) tags and 139,176 high-quality DNA polymorphisms were identified using specific length amplified fragment sequencing (SLAF-seq), including 132,880 SNPs and 6,296 InDels between the reference genome (TM-1) and the five tetraploid cotton species.

SUBMITTER: Shen C 

PROVIDER: S-EPMC5363057 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature