CLARITY reveals dynamics of ovarian follicular architecture and vasculature in three-dimensions.

ABSTRACT: Optimal distribution of heterogeneous organelles and cell types within an organ is essential for physiological processes. Unique for the ovary, hormonally regulated folliculogenesis, ovulation, luteal formation/regression and associated vasculature changes lead to tissue remodeling during each reproductive cycle. Using the CLARITY approach and marker immunostaining, we identified individual follicles and corpora lutea in intact ovaries. Monitoring lifetime changes in follicle populations showed age-dependent decreases in total follicles and percentages of advanced follicles. Follicle development from primordial to preovulatory stage was characterized by 3 × 10⁵-fold increases in volume, decreases in roundness, and decreased clustering of same stage follicles. Construction of fol

SUBMITTER: Feng Y 

PROVIDER: S-EPMC5363086 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature