Isolation and characterization of human articular chondrocytes from surgical waste after total knee arthroplasty (TKA).

ABSTRACT:

Background

Cartilage tissue engineering is a fast-evolving field of biomedical engineering, in which the chondrocytes represent the most commonly used cell type. Since research in tissue engineering always consumes a lot of cells, simple and cheap isolation methods could form a powerful basis to boost such studies and enable their faster progress to the clinics. Isolated chondrocytes can be used for autologous chondrocyte implantation in cartilage repair, and are the base for valuable models to investigate cartilage phenotype preservation, as well as enable studies of molecular features, nature and scales of cellular responses to alterations in the cartilage tissue.

Methods

Isolation and consequent cultivation of primary human adult articular chondrocytes from the surgical w

SUBMITTER: Naranda J 

PROVIDER: S-EPMC5363257 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature