A Synthetic Mammalian Therapeutic Gene Circuit for Sensing and Suppressing Inflammation.

ABSTRACT: Inflammation, which is a highly regulated host response against danger signals, may be harmful if it is excessive and deregulated. Ideally, anti-inflammatory therapy should autonomously commence as soon as possible after the onset of inflammation, should be controllable by a physician, and should not systemically block beneficial immune response in the long term. We describe a genetically encoded anti-inflammatory mammalian cell device based on a modular engineered genetic circuit comprising a sensor, an amplifier, a "thresholder" to restrict activation of a positive-feedback loop, a combination of advanced clinically used biopharmaceutical proteins, and orthogonal regulatory elements that linked modules into the functional device. This genetic circuit was autonomously activated by inflamm

SUBMITTER: Smole A 

PROVIDER: S-EPMC5363301 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature