Codon Optimization Leads to Functional Impairment of RD114-TR Envelope Glycoprotein.

ABSTRACT: Lentiviral vectors (LVs) are a highly valuable tool for gene transfer currently exploited in basic, applied, and clinical studies. Their optimization is therefore very important for the field of vectorology and gene therapy. A key molecule for LV function is the envelope because it guides cell entry. The most commonly used in transiently produced LVs is the vesicular stomatitis virus glycoprotein (VSV-G) envelope, whose continuous expression is, however, toxic for stable LV producer cells. In contrast, the feline endogenous retroviral RD114-TR envelope is suitable for stable LV manufacturing, being well tolerated by producer cells under constitutive expression. We have previously reported successful, transient and stable production of LVs pseudotyped with RD114-TR for good transduction of

SUBMITTER: Zucchelli E 

PROVIDER: S-EPMC5363313 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature