Improved cerebral energetics and ketone body metabolism in db/db mice.

ABSTRACT: It is becoming evident that type 2 diabetes mellitus is affecting brain energy metabolism. The importance of alternative substrates for the brain in type 2 diabetes mellitus is poorly understood. The aim of this study was to investigate whether ketone bodies are relevant candidates to compensate for cerebral glucose hypometabolism and unravel the functionality of cerebral mitochondria in type 2 diabetes mellitus. Acutely isolated cerebral cortical and hippocampal slices of db/db mice were incubated in media containing [U-¹³C]glucose, [1,2-¹³C]acetate or [U-¹³C]β-hydroxybutyrate and tissue extracts were analysed by mass spectrometry. Oxygen consumption and ATP synthesis of brain mitochondria of db/db mice were assessed by Seahorse XFe96 and luciferin-lucifer

SUBMITTER: Andersen JV 

PROVIDER: S-EPMC5363491 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature