The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides.

ABSTRACT: Cell-penetrating peptides (CPPs) are delivery vectors widely used to aid the transport of biologically active cargoes to intracellular targets. These cargoes include small interfering RNAs (siRNA) that are not naturally internalized by cells. Elucidating the complexities behind the formation of CPP and cargo complexes is crucial for understanding the processes related to their delivery. In this study, we used modified analogs of the CPP transportan10 and investigated the binding properties of these CPPs to siRNA, the formation parameters of the CPP/siRNA complexes, and their stabiliy to enzymatic degradation. We conclude that the pH dependent change of the net charge of the CPP may very well be the key factor leading to the high delivery efficiency and the optimal binding strength between

SUBMITTER: Parnaste L 

PROVIDER: S-EPMC5363680 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature