Acute changes in neurovascular reactivity after subarachnoid hemorrhage in vivo.

ABSTRACT: Subarachnoid hemorrhage causes acute and long-lasting constrictions of pial arterioles. Whether these vessels dilate normally to neuronal activity is of great interest since a mismatch between delivery and consumption of glucose and oxygen may cause additional neuronal damage. Therefore, we investigated neurovascular reactivity of pial and parenchymal arterioles after experimental subarachnoid hemorrhage. C57BL/6 mice were subjected to subarachnoid hemorrhage by filament perforation or sham surgery. Neurovascular reactivity was assessed 3 h later by forepaw stimulation or inhalation of 10% CO₂ Diameters of cerebral arterioles were assessed using two-photon microscopy. Neurovascular coupling and astrocytic endfoot Ca²⁺ were measured in brain slices using two-photon and

SUBMITTER: Balbi M 

PROVIDER: S-EPMC5363735 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature